Biologists use FFU laminar flow hood to prepare agar plate

Molecular biology and bacteriology use agar plates to culture microorganisms, such as bacterial experiments Agar coagulating agent, or gel, is the nutrient medium needed for liquid microorganism to survive and grow Once transferred to an agar plate, the bacteria grow into different colonies (if growing at low concentrations) or smears (if at high concentrations) and can be harvested for analysis Biologists prepare agar plates, a process that requires access to laboratory grade reagents and a good understanding of machine and molecular biology and aseptic techniques 1 Wear appropriate protective equipment, including gloves, laboratory clothes, masks, and clean all surfaces Working in Bunsen burner, Zui is good at being able to work in a sterile FFU laminar flow hood This prevents air pollutants from falling into agar 2 Remove the container from the stack except on the repacked agar plate Label this dish on the outer surface (LID) and agar type and date of preparation The plate comes out as a separate layer with the lid removed to simplify the pour of agar 3 Mix tryptone, yeast extract and agarose together, then add sodium hydroxide (sodium hydroxide) solution Add sterile distilled water to obtain 1 l nutrition medium This solution is transferred to a glass bottle with a volume of at least 1.5 liters Loosen the screw cover of the bottle and label the date of preparation and media name Autoclave tape in bottle and autoclave for 25 minutes Allow the bottle to cool to not less than 45 ° C (or to harden prematurely) but not more than 50 ° C The agar can be cooled and coagulated in a storage bottle at 4 ℃ and then heated until liquefied If you need specific experiments or strains of bacteria, add the right type and amount of supplements, such as antibiotics If the media is too hot, the supplements will be destroyed, so check the temperature before the media 4 Pour a sufficient amount of agar half the plate while the agar is still warm and liquefiable Make sure the surface is completely covered Do not overdo or peak the agar of this dish Keep plates in a sterile atmosphere by allowing Bunsen burner or laminar flow hood to run continuously Allow the plate to set and then replace the cover 5 Dry the agar plate for 30 minutes to an hour in the oven at no higher than 37 ℃ Alternatively, you can have them in the FFU laminar flow hood for three minutes or in the low flow area of the purification laboratory, which is not ventilated, for a day or two Dry plate stop any bacterial solution placed on agar surface from simply diluting and sliding, instead of forming colonies, adhere to agar 6 Carefully store the disk in the dark, at 4 degrees Celsius Stack dry agar plate with a dry cup to let you clearly see the label and prevent microorganisms and debris from falling onto the agar surface Put the plates back in their packaging, the cover and the aluminum tape packaging closed Prepare the packaging board with their content and date labels You can store disks for up to two months and should discard them.