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Bioluminescent Assay of the Adenylate Energy Charge

 Last Update: 2020-12-29
 Source: Internet
 Author: User

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The “adenylate control hypotheses” proposed by Atkinson from UCLA (
1
) was further developed into the adenylate energy charge (AEC) concept in the 1960s (
2
,
3
) and thereafter codified (
4
). This concept arose from making an analogy between the adenme nucleotide pool of ATP, ADP, and AMP, and a chemical storage battery or accumulator cell. The common elements include: (1) the total amount of material typically remains constant, and (2) chemical energy can be stored and recovered by alteration of the ratio of the components. The charge of the three-component adenylate system is given by the mole fraction of ATP plus 1/2 of the mole fraction of ADP. It is shown in the following expression:

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