BIOPIC Bai Fan's research team and collaborators reveal the co-evolutionary characteristics of Epstein-Barr virus-positive gastric cancer tumor cells and Epstein-Barr virus

Gastric cancer ranks third in the global mortality rate, and patients usually have a five-year survival rate of less than 40%

September 8, 2021, biological forefront of innovation Medical Center (BIOPIC), Peking University, Beijing future genetic diagnosis sophisticated Innovation Center (ICG) white where fellow Task Force in cooperation with the Cancer Center of Sun Yat Chung Sin research group in Genome Medicine magazine Published a paper titled " The genomic architecture of EBV and infected gastric tissue from precursor lesions to carcinoma " in the form of a long research article

Figure 1.

Through whole-exome sequencing, the researchers found that EBVaGCs and high-grade dysplasia (HD) in precancerous lesions are more effective than normal gastric mucosal tissue and low-grade dysplasia (LD).

Figure 2.

The researchers further performed clonal analysis on all precancerous lesions and tumor samples of each patient in the cohort, and found that the EBVaGCs of each patient were of monoclonal origin by constructing a phylogenetic tree, and all HDs had the same origin as EBVaGCs (Picture 3)

Figure 3.

The researchers further analyzed the EBV genome

Hypermethylation is a significant molecular feature of EBVaGCs.

Figure 4.

By integrating molecular events at different stages of tumor development, the researchers found that EBVaGCs and HD have high-frequency PI3K-Akt pathway genes (PIK3CA/B, PTEN), Wnt pathway genes (CTNNB1, GNAS) and ARID1A mutations and copy numbers Mutations, and these mutations are rare in normal gastric mucosal tissues and LD

Figure 5.

Dr.