Biosafety cabinet of main laboratory biosafety equipment

1、 Overview of biosafety cabinet (1) concept: biosafety cabinet (BSC) is a negative pressure exhaust cabinet designed to protect operators, laboratory environment and experimental materials from exposure to infectious aerosols and splashes generated in the process of operation when operating infectious materials （2） Selection of biosafety cabinet protection type protection object of biosafety cabinet class I Class II, III and IV pathogenic microorganisms; operators of volatile radionuclides / chemicals, environmental class II A1 type II, class II, III and IV pathogenic microorganisms Operator environment and sample class II, type A2, class II, class III and class IV pathogenic microorganisms; trace volatile radioactive nuclides / chemicals operator environment and sample class II Class B1, II, III and IV pathogenic microorganisms; a small number of volatile radionuclides / chemicals operators, environment and samples class II, type B2 Class II, III and IV pathogenic microorganism; volatile radionuclide / chemical operator, environment and sample III class I, II, III and IV pathogenic microorganism; volatile radionuclide / chemical Operator, environment and sample 1 Level I biosafety cabinet - level I biosafety cabinet can provide protection for personnel and environment, but does not provide product protection Use: class I biosafety cabinet is used as special sealing equipment (such as centrifugation, harvesting equipment or small fermentation equipment) or operation process that may produce aerosol (such as garbage pouring into cage, aeration culture or tissue agitation) It can also be used to operate radionuclides and volatile toxic chemicals It has been replaced by medical air isolation device with higher safety protection performance 2 Class II biosafety cabinet -- class II biosafety cabinet can provide protection for personnel, environment and products Purpose: to carry out biosafety level 1, 2 and 3 related operations 3 Level III biosafety cabinet - level III biosafety cabinet is a airtight structure without an open observation window; it provides Zui protection for the environment, products and operators Purpose: to operate microbial pathogens with level 4 biosafety 2、 The use of biosafety cabinet (I) personal protection requirements: ① when using the biosafety cabinet, you must wear personal protective clothing and gloves (gloves should be put on the outside of the isolation clothing); ② Zui should wear a mask and hat, and wear a protective eye mask or mask as required ③ Before work, the operator should adjust the height of the stool to ensure that his face is above the opening （2） The principle of placing the articles in the biosafety cabinet: ① place them from the clean area to the polluted area ② All items should be placed as far away from the front grille as possible, and close to the rear edge of the console ③ Put the waste bag, the tray containing the waste suction pipe and the suction filter bottle and other larger items on one side of the cabinet ④ The front grille shall not be blocked by the experimental record book, instruments and equipment or other articles (3) the operation process of the biosafety cabinet: Turn on the power switch of the exhaust fan outside the biosafety cabinet -- slowly open the operation window, the opening distance of the window is 200 mm or the set value, the general biosafety cabinet has the window limit alarm -- the experimental operation can be started after 10 minutes -- pay attention to the disinfection and cleaning of the biosafety cabinet after the operation is completed (IV) precautions for the use of the biosafety cabinet-- ① The safety cabinet can only be used when it works normally ② Slowly and vertically enter the front opening with both arms, and wait for about one minute in the biosafety cabinet to adjust the cabinet before handling the items ③ The waste shall be discarded in the treatment container in the biosafety cabinet to avoid rapid movement of arms at the front opening and frequent access, so as to avoid interference with the air flow ④ Try to avoid polluting objects entering the clean area ⑤ When operating in the biosafety cabinet, it is not allowed to carry out literal work ⑥ All work in the cabinet shall be carried out in the middle or rear of the working face and can be seen from the observation panel ⑦ the front feed style grid shall not be blocked by notes, droppers, or other materials, otherwise it will interfere with the air flow and may contaminate the materials and expose the personnel ⑧ Cleaning and disinfection: after each work and at the end of the day, the surface of the safety cabinet shall be cleaned with a suitable disinfectant ⑨ the fan of the cabinet shall run for another 5 minutes before and after the work III super clean bench the super clean bench is designed to protect the test sample, and prevent the test sample from being polluted by dust or bacteria by blowing the laminar air in the working area Infectious materials can not be operated in ultra clean worktable, because the gas is directly discharged under positive pressure Once the infectious sample is placed in the working area, the laminar air will blow the air with microbial medium to the working personnel at the front desk, which will cause danger The difference between biosafety cabinet and super clean working table Biosafety cabinet, complex negative pressure, discharged after purification, operator's sample environment, super clean workbench Simple positive pressure direct discharge sample in addition to understanding the performance indicators of the safety cabinet, Zui good Zui quick Zui convenient way to identify the safety performance of the safety cabinet is to understand the biology you purchased Whether the safety cabinet has obtained the following major international safety cabinet certification: nsf49 (American biosafety cabinet standard - Secondary biosafety cabinet), en12469 (European biosafety cabinet unified standard), etc