Biosafety cabinet, ventilation cabinet and super clean workbench

A clear understanding of the distinction between biosafety cabinets and fume hoods / ultra clean benches is required The fume hood and super clean bench are not biosafety cabinets and cannot be used in the experiment or production process involving microbial materials Biological safety cabinets (BSCs) are designed to protect workers, laboratory environment and experimental products from exposure to infectious aerosols and splashes that may be generated during the above operations when operating infectious experimental materials such as primary cultures, bacterial strains and diagnostic specimens The laboratory biosafety manual published by who clearly states that "biosafety cabinet can effectively reduce laboratory infection and culture cross contamination caused by aerosol exposure, while biosafety cabinet can also protect the working environment" Of course, the premise of effective work of safe cabinet is to choose qualified safe cabinet and use safe cabinet correctly The fume hood (fume hood) is designed to remove corrosive chemical gases and toxic fumes during chemical experiments As there is no HEPA filter, the fume hood can not effectively remove the microbial media Microbial samples placed in the fume hood will be spread outside the fume hood, polluting the laboratory environment Ultra clean bench (ultra clean bench) is designed to protect the test article or product It can prevent the test article or product from being polluted by dust or bacteria outside the work area by blowing the vertical or horizontal laminar air of the work area Once the microbial sample is placed in the working area, the laminar air will blow the air with microbial medium to the front desk staff, which will cause danger Attachment: the basic certification knowledge of biosafety cabinet and super clean platform is not well understood by most users on the performance, testing and testing of the cabinet and super clean platform, which results in a blind area for understanding these instruments But sometimes these blind spots can cause fatal injuries, such as the virus leakage in Zui's laboratories near Singapore, Taiwan and Beijing It is not enough to measure the safety of super clean platform or safe cabinet only by wind speed The standard inspection and certification rules ensure the reliability of the inspection and the safety performance of the safety cabinet At present, the international standards of super clean platform are: air cleanliness level meets iso14644.1; Australian standard as 1807; iest-rp-cc002.2 Biosafety cabinet standard: American ANSI / nsf49 (secondary cabinet); European standard en12469:2000 (primary, secondary and tertiary cabinet); super clean platform can only protect samples, but not operators, and its certification includes the following steps: 1) measure the air flow rate of super clean platform with rotating impeller anemometer and heat anemometer The average value of safe air flow: 0.4-0.5m/s, Zui big deviation 20% 2) Measure the integrity of ultra clean filter: use natural wetting agent detector 3) Measure the internal light intensity and noise of super clean platform Biosafety cabinet is closely related to scientific research at present, which includes two-level biosafety cabinet and three-level biosafety cabinet There are several types of secondary biosafety cabinets, but the type A / B3 with 30% gas discharge, 70% gas circulation and type B2 with 100% gas discharge are often used in the laboratory Biosafety cabinet can not only protect samples but also operators, so it is called "safe" cabinet The detection steps are as follows: 1) measure the inflow air flow (forward air flow): use a heat anemometer or dim hood 2) Measurement of downward air flow: according to nsf49 and en12469 standards, there are two ways But they all use heat anemometers to measure the down flow rate 3) ULPA filter detection: wet agent photometer, wet agent generator, pressure measuring instrument, Pao measuring instrument 4) There are also noise, light intensity, vibration intensity measurements 5) After Zui, the smoke generator was used to test the air flow visualization Shanghai Sujing Industry Co., Ltd if you have any need, you can pass with us at any time Thank you very much for your support and look forward to cooperating with you Thank you & bestragard! Suing Jinghua