Biosci Rep: Hexa-yellow pills can be used to treat osteoporosis associated with diabetic nephropathy.

The effects of LWDH on diabetic nephropathy-related osteoporosis (DNOP) are unclear.
study was conducted to evaluate the effects of LWDH on KDM7A and Wnt/β-catenin signaling path pathps in DNOP rats and high-sugar-induced MC3T3-E1 cells, the results of which were published online in Biosci Rep.
studied 9-week-old male Sprague-Dawley (SD) rats and prepared a DNOP model with strepzozolethatin to evaluate the effects of LWDH.
the cell vitality and differentiation of high-sugar-induced MC3T3-E1 cells were measured by CCK-8, Alizarin red staining and alkaline phosphatase (ALP) staining, respectively.
addition, the expression of KDM7A and Wnt1/β-catenin path-related proteins was determined by Western blot analysis.
results showed that the treatment of DNOP rats with LWDH significantly improved the general state of rats, the deterioration of kidney function and changes in kidney pathology.
LWDH can reduce the levels of TNF-α, IL-6, IL-8, IL-1 beta, ALP and TRAP, improve the content of calcium and phosphorus in the serum, and reduce the content of calcium and phosphorus in urine.
addition, LWDH significantly improved bone density (BMD), bone mass (BV) and bone microstructage in DNOP rats.
addition, LWDH increases the elastic mould, limit load and bending strength of the collarbone.
in MC3T3-E1 cells, serum-containing LWDH significantly improves cell vitality and osteoblast differentiation.
α-SMA, vimentin, KDM7A, Wnt1 and β-catenin were significantly reduced, and expressions of E-cadherin, H3K9-Me2, H3K27-Me2, BMP-4, BMP-7, Runx2, osteocalcin and Col1a1 were significantly revised up after LWDH treatment.
, the results show that LWDH has therapeutic effects on DNOP, in part by lowering the KDM7A and Wnt/β/catenin paths.
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