BONCAT-FACS technology enables rapid identification of soil active microorganisms.

Soil is not only broken mineral rocks and animal and plant residues, but also has a complex microbial population to become the base of crop prosperity.
without little guys such as fungi and bacteria to break down macromolecules, the soil would not be fertile, and a particular plant would have a microbial ecosystem to symbiosis.
new technology for rapid micro-screening, it will make it easier for humans to get a faster grasp of the soil conditions in a particular area, whether it's protecting microsystems or growing suitable crops.
researchers at the U.S. Department of Energy's Lawrence Berkeley National Laboratory, Oak Ridge National Laboratory and other institutions published a study in the journal Nature Communications on the 24th, they used a technique called BONCAT-FACS, in a few hours to successfully identify the presence of active microorganisms in soil samples.
researchers say the study could change the current paradigm of scientists' research on soil microbes, which is important for sustainable agricultural development and environmental engineering research.
BONCAT, an acronym for "bioorthogonal non-standard amino acid markers," was invented in 2006 by geneticists at the California Institute of Technology to isolate newly manufactured proteins in cells.
Energy Department researchers have improved this approach by developing BONCAT-FACS (fluorescent-activated cell sorting) technology that allows them to classify single-celled organisms based on the presence or absence of fluorescently labeled molecules.
new technology is not only more simplified, reliable and time-saving than previous microbial identification methods, and the whole process takes only a few hours, which is an effective means of soil microbiological research.
the researchers used the method to measure microbial population activity in two soil samples in Oak Ridge, Tennessee, and successfully isolated the active microorganisms present in the samples.
soil contains the most diverse microbial community on Earth.
there are thousands of microorganisms in every gram of soil that are the backbone of terrestrial ecosystems and the key to sustainable agricultural development.
But because most soil microbes cannot grow in laboratory cultures, it is difficult to study the activity and interaction of these microorganisms.
more than 500 years ago, Leonardo da Vinci pointed out that humans "know more about celestial motion than the soil beneath their feet".
today, soil microbiology is still an important research topic facing scientists.
new research shows that BONCAT-FACS is an effective tool that can help scientists uncover the relationship between soil processes and specific microbial populations.
researchers say that using BONCAT-FACS technology to effectively detect soil microbial activity components in situ and determine which microbes are active at a specific time and which are dormant, help scientists gain insight into how microbes respond to normal habitat fluctuations and extreme weather events such as droughts and floods, which in turn drive soil microbiome research.
is of great significance to the use of microorganisms to improve agricultural land, improve crop resistance and promote the development of environmental engineering.
the soil microbial species: soil micro-organisms is a mirror of soil ecological environment, by analyzing the relevant situation of soil microorganisms to help us understand the soil and biological chain process on which we depend, for soil micro-organisms, what do you know? Soil microbiome refers to the types, quantities and metabolic activity intensity of participating substance strains in soil under a particular environmental and ecological condition.
in studying microbial systems, it should be noted that no medium or culture condition can simultaneously produce all microbial species in the soil.
any medium is a selective medium, except that the selection range and object of the various media are different.
it is therefore necessary to use a variety of selectively high mediums to determine the number of specific physiological groups in the soil.
applied molecular biology techniques show that the traditional methods of microbiology to separate culture of culture accounted for only about 1% of the total number of environmental microbial species such as soil, and a large number of unknown species are still uncultivated.
the characteristics of different soil microbiomes can reflect the comprehensive characteristics of soil ecological environment.
can reflect the degree of soil maturation and ecological environment.
such as garden brown nitrogen fixation bacteria can be used as an indication of soil maturation, basically in a variety of raw soil can not be separated, and after farming soil can be separated, and the longer the farming life, the more the number of garden brown nitrogen bacteria per g of soil. the advantages of
fibrosis bacteria are different in different degrees of maturation of soil.
is mainly clump mold in raw soil, in soil with strong organic matter mineralization and high nitrogen content, mainly woolen mold and sickle mold, in the cooked soil the dominant bacteria are sacs stick bacteria and spore-eating fiber bacteria, and in soil with organic fertilizer and inorganic nitrogen fertilizer, fibrosis and fibromytosis are the dominant bacteria. The types, quantities and activity intensity of microorganisms in the soil microbiome of
have strong annual cycles with seasonal changes, including temperature, humidity, and the entry of organic matter.
according to the development al-strain of soil microorganisms in the soil, can be divided into indigenous and fermentation zones two categories: indigenous microbiomes are those that are not sensitive to fresh organic matter, maintained at a certain quantitative level, even if the organic matter is added or temperature, humidity changes in quantity, the change is also small.
such as Gram-positive cocci, bacillus, bacillus spores, bacillus, mycobacteria, line bacteria, penicillin, courmic, clumps and so on.
fermentation microbiomes are those that are sensitive to fresh organic matter and develop explosively when there are fresh animal and plant residues, and those that fade quickly after the disappearance of fresh residues.
include all kinds of Gram-negative spores, yeast, as well as Bacillus spores, streptomy, root mold, citamy, wood mold, sickle mold and so on. The number of
fermentation microbiome varies greatly.
therefore, when there are fresh organic residues in the soil, the fermentation microorganisms have a large number of development advantages, while the fresh organic residues are decomposed, the fermentation microorganisms decline, the indigenous microorganisms have the advantage.
Source: Science and Technology Daily, Beina Bio.