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Tags:Cryovials Storage tubes Storage tubes Cryovials Use steps (1) Fresh cultures are selected from the pure bacterial culture to form a bacterial suspension inoculation and culture preservation tube with a turbidity of about 3-4 Wheat
.
(2) Tighten the preservation tube, reverse it back and forth 4-5 times to emulsify the bacteria, and do not shake
.
(3) Put the preservation tube into the refrigerator for storage (-20°C--70°C) Recommendations for the safe use of cryovials 1.
When using cryovials to store samples, it is strictly required that the cryovials should be stored in the vapor layer of liquid nitrogen or in the refrigerator! If the cryovials are stored in liquid nitrogen liquid, liquid nitrogen has a certain chance of seeping into the inside of the cryopreservation tube, and the vaporization of liquid nitrogen during resuscitation leads to unbalanced pressure inside and outside the tube, which is very easy to cause the cryopreservation tube to burst and is biohazardous
.
2.
Operate the cryovialization tube to recover, use safety protective equipment throughout the process, it is recommended to wear experimental coats, cotton gloves and operate
on a safe experimental bench.
Wear goggles or face shields
when possible.
Since the indoor temperature will be higher in summer than in winter, please be extra careful
.
3.
During the storage of cryopreserved cells, the freezing temperature of the cryopreservation tube must be uniform.
Uneven freezing can lead to the creation of ice plugs, which inhibit the temperature transfer of liquid on both sides, creating dangerously high pressures and damaging
the cryovials.
4.
The sample volume of cryopreservation should not exceed the maximum working volume
required by the cryopreservation tube.
Summary: The use of cryovials and precautions, Bunsen biology editor will share here, after reading this article you should have a basic understanding and understanding I believe everyone understands! Overall, hope it helps
.
