Cai Huaqing's research group reveals the molecular mechanism of macropinosome maturation

On April 4, 2022, Huaqing Cai's group from the Institute of Biophysics, Chinese Academy of Sciences published a research paper titled "The PripA-TbcrA complex-centered Rab GAP cascade facilitates macropinosome maturation in Dictyostelium " in the journal " Nature Communications " , which revealed that giant cells Molecular mechanism of the regulation of macropinosome maturation and cargo degradation by the Rab GAP signaling cascade composed of Rab5, Rab7, PripA and TbcrA during body maturation .

Macropinocytosis is the non-selective endocytosis of extracellular fluid by cells

In order to analyze the molecular mechanism of the maturation process of macropinosomes, Huaqing Cai's group used the model organism Dictyostelium discoideum cells to conduct research

Further through the screening of proteins specifically localized to macropinosomes during the transformation stage of Rab5 to Rab7, a protein complex composed of a protein containing a PH domain and a protein containing a RabGAP domain was found, and the The two proteins were named PripA and TbcrA, respectively

Taken together, this study revealed the molecular mechanism by which Rab5, Rab7 and PripA-TbcrA complexes regulate macropinosome maturation in the form of a Rab GAP cascade

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Tu Hui, a doctoral student at the Institute of Biophysics, Chinese Academy of Sciences, is the first author of this paper, and researcher Cai Huaqing is the corresponding author of this paper

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(Contributed by: Cai Huaqing Research Group)