Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprinting Spectra
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Last Update: 2021-03-05
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Source: Internet
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Author: User
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This chapter describes a number of aspects important for calibration of matrix-assisted laser desorption/ionization time-of-flightspectra prior to peptide mass fingerprinting searches. Both multipoint internal calibration and mass defect-based calibrationis illustrated. The chapter describes how potential internal calibrants, like tryptic autodigest peptides and keratin-relatedpeptides, can be identified and used for high-precision calibration. Furthermore, the construction of project/user-specificlists of potential calibrants is illustrated.
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