Canine cyclophosphate adenosine (cAMP) enzyme-linked immunology analysis

< "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt; COLOR: rgb (0,0,0); "> Dogscyclic adenosine ( cAMP)ase-immune < "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt; "> analysis

reagents< "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt;" > box instruction manual

reagents are for research purposes onlyPurpose: This kit is used to determine dogserum plasma and related liquid samplesadenosine cyclophosphate (cAMP)content.

Experimental Principles :

kits are measured using a dual antibodysandwich method < span style: "FONT-SIZE: 10.5pt;" > specimen < "FONT-SIZE: 10.5pt;" > indogAdenosine cyclophosphate(cAMP)Level. With purifieddog adenosine cyclophosphate (cAMP) antibody package is microplate, made into solid-phase antibodies, into the packet of monoantigen micro-holes in turn added adenosine cyclophosphate (cAMP) , and HRP mark phosphate adenosine (cAMP) antibody binding, forming antibodies- < a href"" > antigen - enzyme-labeled antibody complex , after thorough washing plus " TMB color. TMB at HRP enzyme catalytic conversion to blue, and under the effect of acid into the final yellow. The shade of color and the adenosine cyclophosphate (cAMP) is positively related. Measure the absorbent at 450 nm wavelength" ( OD value), through the standard curve calculated > samples < adenosine cyclophosphate (cAMP) concentration.

kit composition < "FONT-SIZE: 10.5pt;">: