Capillary Electrophoresis for the Analysis of Glycosaminoglycan-Derived Disaccharides
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Last Update: 2021-01-03
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Source: Internet
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Author: User
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Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its highresolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupledto laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detectionat 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive amination,which is one of the most important method of derivatization of disaccharides for CE-LIF detection. Outlined in this chapteris a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.
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