echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels

    Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels

    • Last Update: 2020-12-03
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    Capillary electrophoresis (CE) in all its various modes is routinely used in the area of life science for the analysis of wide spectra of species ranging from small inorganic/ organic molecules to multi-subunit proteins and mega-size
    DNA
    (
    1
    ). Capillary gel electrophoresis (CGE) is the counterpart of gel electrophoresis in the slab-gel format. Similar molecules are analyzed with CGE as with slab gels, like
    SDS
    -protein complexes and single-stranded/duplex DNA. Since these analytes have similar mass-overcharge ratio and therefore similar mobilities (for ≥10 bases in length, depending on pH and base composition) they can not be separated in a carrier-free system like capillary free-zone electrophoresis (
    2
    ), although some exceptions are known (
    3
    ,
    4
    ). A size-sieving medium is therefore necessary to impart hydrodynamic friction on the migrating molecules for their separation. The constraint of the capillary format also acts to prevent convection, whereas in the slab-gel format the sieving matrix plays this role.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.