Carnations are fast and complex

equipment and
reagents ultra-clean workstation
, complete set of
cultured
dishes with absorbent paper, sterile waterficial
N
6
1 mg/L 6-BA
Scissors, gun-type tweezers meter
, alcohol lamp, filter paper, distilled water, small brush
95% ethanol, 70% alcohol, 1 70% alcohol cotton balls, 0.2% liters of mercury, formaldehyde, potassium permanganate, rasool
gauze, cotton plugs, krap paper, pure, soap, alcohol spray pot
plant stalks, side buds, stem tips, Leaves, flowers, healing
tissues
on the infinitive buds, embryos,
test tubes
seedlings, etc.
2, experimental material carnation branches
iii, methods and steps
1 explant sterilizationTake carnation branches purchased from the market, remove large leaves, cut knots with buds, wash the surface with soap (cleanser), rinse with tap water for 30-60 minutes, and then soak 70% alcohol in the sterile chamber (ultra-clean work table) for 20-40 seconds (according to the degree of woody material to grasp the specific immersion time). Soak for 10-15 minutes with a 0.2% mercury-boosting solution, rinse with sterile water 3-4 times, and place on filter paper in a sterilized petri dish for use.
2 vaccination first for sterile indoor disinfection, with ultraviolet lamps for 30-45 minutes, the ground with a low concentration of insul solution disinfection, UV lights off about 20 minutes before going in to work.
clean work table disinfection to turn on the sterile wind switch, so that the sterile wind blows on 30-45 minutes before working. And wipe the work table with 70% alcohol cotton balls.
light the alcohol lamp when inoculated, and soak the tweezers and scissors in 70% of the alcohol solution first. Use the alcohol lamp to burn the cooled tweezers, scissors to remove a side bud or small stem, quickly open the triangular bottle mouth, the material is inserted into the bottle, pay attention to the polarity of the upper and lower ends of the material, can not be inverted. Plug back tin foil at the edge of the alcohol lamp and write the date and material on the bottle body with a marker pen.
3 Culture conditions: 25 degrees C light 13 hours / day light strength 1000 Lux
4 step-by-generation culture
5 induced root: with low concentrations of pyridine or acetic acid or no
hormone
N
6
culture to induce the root
6 test tube seedlings transplanted