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Catharanthus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids

 Last Update: 2020-11-17
 Source: Internet
 Author: User

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A protocol for the establishment of in vitro shoot cultures of
Catharanthus roseus
is described. Shoots can be maintained for more than 1 yr without evidence of tissue vitrification, disaggregation, or callus formation. Vindoline was the main alkaloid accumulated, reaching values similar to those found in leaves from field-grown plants, after a long period of culture. An induction methodology to reduce such waiting time is also presented.

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