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Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice

 Last Update: 2021-03-12
 Source: Internet
 Author: User

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stem cells used

transmitted light microscopy

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Major advances have been made in recent years in our understanding of anther development through a combination of genetic studies, cell biological technologies, biochemical analysis, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize the widely used protocols for pollen viability staining; the investigation of anther morphogenesis by light microscopy of semi-thin sections; TUNEL assay for programmed tapetum cell death; and laser microdissection procedures to obtain specialized cells or cell layers for carrying out transcriptomics.

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