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Cell-Free Protein Synthesis for Analysis by NMR Spectroscopy

 Last Update: 2020-12-13
 Source: Internet
 Author: User

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protein purification protocol

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Cell-free protein synthesis offers fast and inexpensive access to selectively isotope labeled proteins thath can be measured by
NMR
spectroscopy in the presence of all the unlabeled proteins in the reaction mixture. No chromatographic purification is required. Using an extract from
Escherichia coli
in a simple dialysis system, the target protein can be prepared at a typical concentration of about 1 mg/ml, which is sufficient for subsequent analysis by NMR. This chapter describes in detail the protocol used in the authors' laboratory.

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