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    Home > Medical News > Latest Medical News > Cell: Japanese scholars make mice transparent and realize the ultimate dream of system biology

    Cell: Japanese scholars make mice transparent and realize the ultimate dream of system biology

    • Last Update: 2014-11-12
    • Source: Internet
    • Author: User
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    Scientists from RIKEN and Tokyo University, Japan, have combined tissue decolorization with light cut fluorescence microscopy to image a variety of organs and even the whole organism, and have obtained extremely detailed internal images The results are published in this issue of cell magazine Understanding the way life works has always been the ultimate dream of systems biology It will be a new way to realize this dream to make tissue and organism transparent and then make accurate imaging of single cell resolution In this study, the researchers used a method called cubic (clear, unobstructed brain imaging cocktails and computational analysis), which they had previously used to image the whole brain Brain tissue rich in lipids is easier to be transparent, but other parts of the body contain many chromophores that can absorb light, such as heme Heme, an important component of hemoglobin, exists in most tissues of the body and blocks light When the researchers studied this problem, they found that aminoalcohol in cubic reagent can extract heme from hemoglobin, which can significantly improve the transparency of organs They used this method to treat the brain, heart, lung, kidney, liver of mice and even the whole young and adult mice, and got the mice with abnormal transparency On this basis, a very clear 3D image is obtained by light sheet fluorescence microscopy To test the utility of this method, the researchers compared the pancreas of diabetic and non diabetic mice and found that there was a significant difference between their islets, which are the structure of pancreas to produce insulin Cubic requires reagents to fix tissues, so it cannot be used in living organisms However, cubic can help people understand the 3D structure of organs and the expression of specific genes in different tissues, said Kazuki Tainaka, the first author of the article "Our method can get almost all transparent young mice and adult mice It's a basic challenge in biology and medicine that people can observe the cellular network in tissues "Cubic can be used for 3D pathological research, anatomical research and immunohistochemical analysis of the whole organism For example, cubic can be used to observe the development of embryos at the cellular level, or the development of cancer and autoimmune diseases An in-depth understanding of the disease process will help us to develop new treatment strategies Whole body imaging at the single cell level is expected to be the ultimate dream in systems biology, "said Hiroki Ueda, leader of the study The team is further improving microscopic imaging methods to quickly image the entire body of adult mice and even the human brain Through this technology, they hope to have a deep understanding of the mechanism of autoimmune diseases and mental diseases  
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