Cell-oriented, precision functional marker research provides new photocontrolled chemical probe tools.

A collaborative study by East China University of Technology and the Shanghai Institute of Medicine of the Chinese Academy of Sciences provides a new photocontrolled chemical probe tool for cell targeting and precision functional markerresearch.
research published online in Nature Communications.
traditional fluorescent probes are susceptible to biobackground light interference and are usually only able to enter the cell through passive diffusion to produce a material identification signal, resulting in low detection accuracy. To solve this problem
, the researchers created a photochromic fluorescent probe that can be used for cell precision positioning and target identification through remote optical control by connecting the stud-mchromatic color-changing molecule, 1,8-pyridine fluorescent fluorescence, with semi-lactose molecules with active targeting function of membrane receptors.
studies have found that the reversible regulation of the probe's stud/part of the flower structure can be achieved by ultraviolet/visible light cycling, and then the circular "on/off" control of the fluorescence emission of probe pyridine can be realized.
, the probe's stud slud state does not interact with the widely present sulphides in the cell, and when the remote light activates its part of the bruising state, the probe can quickly react with sulphate anions, thus blocking the photochromic activity of the probe, so that fluorescence in a constant "open" state.
the researchers further used the construction probe to achieve cell precision fluorescence markers and optical lysing target identification.
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