Chinese scholars: How do key transcription factors manage contact with DNA?

CTCF is a transcription factor that has become the target of research due to its role in regulating a key oncogene called MYC

Biologists believe that CTCF is the main regulator of the genome and seriously affects the three-dimensional structure of the genome

"There is still a gap in our understanding of the direct role of CTCF, which is important for understanding the transcription process," said senior and co-corresponding author Chunliang Li from the St.

New technology leads the trend

The researchers used cutting-edge technology and multidisciplinary methods, using the auxin-induced degradation system

Dr.

The team also studied multiple sets of data, including ATAC-seq, RNA-seq, whole-genome bisulfite sequencing, Hi-C, Cut&Run, and CRISPR-Cas9 screening technologies, as well as time-resolved deep proteomics and auxin-carrying Phosphorus proteomics analysis in cells that induce degradants

"The multidisciplinary collaboration between our teams made these discoveries possible," said author Dr.

Understand the direct impact on CTCF

This study emphasizes that the loss of CTCF reconnects genome-wide chromatin accessibility, which plays a key role in transcriptional regulation

"CTCF is part of the basic mechanism that drives cell activity, but if there is no suitable tool to study it, its direct role has always been controversial," said co-first author Dr.

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DOI

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Article title

Acute depletion of CTCF rewires genome-wide chromatin assembly