Chinese scientists draw the world's first atlas of human cells

a single-cell-level map of human cells Photo Source: Zhejiang University, " This work is generally said to be the digitization of human cells. Guo said they used a digital matrix to describe the characteristics of each cell and classify it systematically. In addition, the researchers found special expression patterns and defined more than 30 new cell species.Talking about the clinical application of spectral maps to medicine, Guo Guoxuan told China Science Daily, "We are now building a database of single-cell transcription groups of normal cells that can be used for the identification of regenerative medical seed cells." In the future, if extended to the disease cell map, it is possible to apply to the diagnosis of the disease's single-cell level. Cell map data are important for understanding the occurrence of disease, but more accumulation is needed for clinical treatment. The
team conducted Microwell-seq high-volume single-cell sequencing analysis of 60 human tissue samples and 7 cell culture samples, systematically mapping human cells spanning two periods of embryo and adulthood, covering eight systems.
this map, the team found that a variety of adult endothyl, endothyl and substring cells appear to act as immune cells in tissues.
In addition, through cross-period, cross-tissue and cross-species cell map analysis, the researchers revealed a universal mammalian cell fate-determining mechanism: stem cells and progenitocyte transcription states are mixed and random, while differentiated and mature cell transcription states are clear and stable, that is, cell differentiation has gone through a process from chaos to orderly development.
For the first time, the study provided a comprehensive analysis of the types of human cells in embryos and adulthood from a single-cell level, and the data will serve as a repository for exploring the mechanisms of cell fate, which will also have an impact on the identification of normal and diseased cell states in the human body.
Guo Guoxuan team used the self-developed Microwell-seq high-volume single-cell analysis platform, established more than 700,000 single-cell transcription group database, identified more than 100 kinds of cells in the human body and more than 800 kinds of cell subsethics. At the same time, the team developed the scHCL single-cell ratio system for the identification of human cell types, and built a blueprint website for human cells.
"Our work has limitations in sequencing depth and cell numbers, but we have a big advantage in data comparability across tissues and species, " said Guo Guoxuan, a professor at the University of Shanghai. The perfect version of the human cell map should also integrate spatial information, multi-group data, and population analysis, which requires the joint efforts of scientists around the world. Guo guo
, said the results stem from the efforts of the team and collaborators over the past two years. Two years ago, the team published the world's first mouse cell map in Cells.
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