Chinese scientists draw the world's first atlas of human cells

, the world's first human cell map was successfully mapped in Zhejiang University.
, March 26, 2020 Beijing time, professor Guo Guoxuan of Zhejiang University School of Medicine published the results online in Nature.
two years ago, the team published the world's first mouse cell map in Cells.
"s work is generally about digitizing human cells. "We can use a numerical matrix to describe the characteristics of each cell and classify them systematically. Special expression patterns were found and more than 30 new cell types were defined. Guo
, the results are mainly due to the team and more than 30 partners in the past two years night after night struggle.Guo Guoxuan (front row) and team member
The paper establishes the basic framework of the human cell map, discovers the widespread immune activation of adult non-immune cells, suggests that the differentiation process of stem cells and ancestral cells in the body is a process of cell gene expression from confusion to order, and believes that the regulators in the genome predetermine the stable state of the differentiation terminal.
Talking about the clinical application of spectra to medicine, Guo Guoxuan told China Science Daily, "We are now building a database of single-cell transcription groups of normal cells that can be used for the identification of regenerative medical seed cells." In the future, if extended to the disease cell map, it is possible to apply to the diagnosis of the disease's single-cell level. Cell map data will be important for understanding the occurrence of disease, but the application of clinical treatment needs more accumulation. The
team performed Microwell-seq high-volume single-cell sequencing analysis of 60 human tissue samples and 7 cell culture samples, systematically mapping human cells spanning two periods of embryo and adulthood, covering eight systems.a single-cell-level map of human cells
through which the team found that a variety of adult endothyl, endothyl and substring cells appeared to act as immune cells in tissues.
In addition, through cross-period, cross-tissue, and cross-species cell map analysis, the team revealed a universal mammalian cell fate-determining mechanism: stem cells and progenitocytes are mixed and random, and differentiated and mature cell transcription becomes clear and stable, that is, cell differentiation underwent a process from chaos to orderly development.
This study is the first comprehensive analysis of embryonic and adult human cell types at a single-cell level, and the data will be a treasure trove of resources to explore the mechanism of cell fate decision, which will have a profound impact on the identification of normal human and diseased cell states.
Guo Guoxuan team used the self-developed Microwell-seq high-volume single-cell analysis platform, established more than 700,000 single-cell transcription group database, identified more than 100 kinds of cells in the human body and more than 800 kinds of cell subsethics.
, the team developed a scHCL single-cell ratio system for the identification of human cell types and built a blueprint website for human cells.
"Our work has limitations in sequencing depth and cell numbers, but we have a big advantage in data comparability across tissues and species, " said Guo Guoxuan, a professor at the University of Shanghai. The perfect version of the human cell map should also integrate spatial information, multi-group data, and population analysis, which requires the joint efforts of scientists around the world. " team
paper information:
DOI:10.1038/s41586-020-2157-4