Chinese scientists propose launching "genome labeling program"

.
"Blind Touch" and "Earth Satellite", li Jinsong, a researcher at the Institute of Bioalysis and Cells of the Chinese Academy of Sciences, presented the two otherwise unrelevant photographs at the 47th Symposium of the Xiangshan Scientific Conference in Shanghai on May 23.
the experts, Li jinsong explained that more than 22,000 encoded protein genes have been revealed since the completion of the Human Genome Project (HGP) in 2003. However, in the post-genome era, progress in proteomic function research has not been satisfactory. He said that the lack of simple and efficient research systems restricts the study of protein function.
", like a blind man touching an elephant, may have made the local situation very clear, hoping to piece together a panorama. "The way genomes are labeled is promising, as is the case with satellite observations of the Earth.
experts proposed that the Genome Labeling Program (GTP) should be launched, which should be led by our scientists.Li Jinsong told China Science Daily that the program will target a single protein in-place label on "artificial sperm cells" with independent intellectual property rights, obtain a genome-wide protein label "artificial sperm cell" library, and then obtain a genome-wide label mouse library of more than 20,000 proteins through egg injection, complete the physiological tissue expression spectrum analysis of more than 20,000 labeled proteins, and create an international model biological center.
In layman's terms, the scientists intend to do this: the first step is to use CRISPR/Cas9 gene editing technology to insert a specific "label" sequence on a specific gene sequence of solitary male monolithic embryonic stem cells called "artificial sperm cells" to obtain "artificial sperm cells" with genetic labels. The second step is to inject the cell into the egg and "artificially inseminate" the mice to obtain the mice that carry the labeled protein. In the third step, the "labeled" gene can be transprined and translated into a protein with a specific label during growth and development in mice.
, as if putting a hat on a protein, scientists can study them in-body, real-time, and dynamically.
, GTP has been the first to obtain the chinese Academy of Sciences strategic pilot B and Shanghai Science and Technology Commission project support, has now set up the Chinese Academy of Sciences Bio-Chemical and Cell Institute GTP research and development center. Scientists have followed these steps to obtain more than 500 labels of "artificial sperm cells" and more than 140 labeled mouse lineages.
" provides a solid foundation for further large-scale implementation. Li Jinsong said.
Jingdong, a researcher at the Shanghai Institute of Nutrition and Health of the Chinese Academy of Sciences, has conducted a study of a protein containing bromine structure (BrD) using GTP "near the water floor". "For the first time, we systematically depict the role of the BRD protein family in maintaining stem cell dryness and self-renewal." She told China Science Daily at the meeting.it is understood that the program will complete a genome range of more than 20,000 "artificial sperm cells" in 5 to 10 years, and access to more than 5,000 important protein label mice, forming an internationally influential experimental animal research and development platform.
scientists are also interested in the future use of GTP in precision medicine.
for example, in neuroscience, quantitative analysis of specific proteins in different nerve tissues, such as the brain, brain, brain trunk and spinal cord, can help scientists understand the distribution of tissue expression in such molecules.
In cancer research, GTP can be combined with tumor metagenetics to track gene expression of transcription factors and modification factors associated with oscic genetic regulation in real time to understand which molecules play a role in cancer development, thus finding new targets for cancer treatment.
in the genetic mechanism of birth defects, GTP has the opportunity to address important issues such as which organs, tissues and cells are involved in the expression of individual pathogenic mutant genes in the body.Li Lin, Executive Chairman of the
Conference, a member of the Chinese Academy of Sciences and a researcher at the Shanghai Institute of Nutrition and Health of the Chinese Academy of Sciences, said: "The GTP program, led by Chinese scientists using independent research and development technology, will usher in a new era of protein research, and we hope that Chinese life scientists will be able to use this platform for the first time to conduct research." ”