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Chloramphenicol Acetyl Transferase Assay

 Last Update: 2021-01-16
 Source: Internet
 Author: User

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The chloramphenicol acetyltransferase
(cat)
gene, isolated from transposon Tn9 of
Escherichia coli
(
1
), is a convenient genetic marker for studies of transformation. (For a detailed description of the CAT gene,
see
Chapter 1 .) In plants, the CAT system is generally used in transient, rather than in stable, gene expression studies. Gene products are detected by assaying crude protein extracts of putatively transformed cells, usually within a few hours of transformation.

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