Chlorogenic acid

Chlorogenic acid

Chlorogenic Acid

【Synonyms】Coffee tannin, 3-caffeoylquinic acid

【Structural formula】

[Molecular formula and molecular weight] C ₁₆ H ₁₈ O ₉ ; 354.

[Source] The dried flower buds of Lonicera ja ponica Thunb

【Properties】Light yellow powder

This product is soluble in hot water

Melting point: 204～206℃

Optical rotation: [α] _D ²⁰ -36°(Cl, H ₂ O)

【Purity Check】

TLC

1.

Developing solvent ethyl acetate - formic acid - water (10:2:3)

Check under UV lamp (365nm) and (254nm)

2.

Developing solvent butyl acetate - formic acid - water (7:2.

Check under UV lamp (365nm) and (254nm)

High performance liquid chromatography

Chromatographic column YWG-C ₁₈ , 10pm (4.

The mobile phase of methanol - water - acetic acid (60:40: 0.

Detection wavelength 326nm

Differential calorimetry

Start temperature 50℃, end temperature 300℃, heating rate 5℃/min

【Structural identification】329,246,220

3400 (-OH), 1708 (C=O), 1660, 1620, 1640, 985, 835

EI-MS m/z: 354[M] ⁺ , 180,163

¹ H-NMR (CD ₃ COCD ₃ ) δ: 2.

¹³ C-NMR (CD ₃ COCD ₃ ) δ: 75.
9(C-1), 37.
7(C-2), 73.
3(C-3), 71.
3(C-4), 71.
1(C-5), 38.
9(C -6), 146.
1(C-α), 114.
9(C-β), 127.
2(C-1'), 116.
2(C-2'), 146.
2(C-3'), 148.
9(C-4'), 115.
3(C-5'), 122.
5(C-6'), 175.
8(C-7), 167.
7(C-8)
.

【Storage】Dry and airtight
.

Related link: Puerarin