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    Home > Biochemistry News > Biotechnology News > Chromosin remodeling factor PKL plays an important regulatory role in the process of RNA-mediated DNA methylation.

    Chromosin remodeling factor PKL plays an important regulatory role in the process of RNA-mediated DNA methylation.

    • Last Update: 2020-08-31
    • Source: Internet
    • Author: User
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    On May 31st, genome biology published online a research paper entitled The Developmental Regulator PKL Is Required to Maintain correct DNA methylation at RNA-directed DNA methylation loci, published online by Genome Biology.
    the study reveals the important regulatory role of chromosin remodeling factor PKL in the process of RNA-mediated DNA methylation.
    In plants, RNA-mediated DNA methylation (RdDM) is an important mechanism for establishing new DNA methylation styles and transcription gene silencing, guiding DNA methyl transfer enzymes to specific points for new DNA methylation through the base pairing of small interfering RNA (siRNA) and stent RNA (scaffold RNA).
    RdDM play an important role in the biological processes such as external gene silencing, maintaining genomic stability, and establishing dna methylation patterns in reproductive cells, and it is important to analyze its molecular mechanism to achieve transcriptional silencing or activation of specific genes.
    study, Zhang's team screened two new mutants involved in GM silencing through positive genetics.
    two mutants were found to be caused by a lack of function of the important developmental regulatory gene PICKLE (PKL).
    whole genome methylation analysis found that PKL affects normal DNA methylation of about half of RdDM sites, with rising and falling sites roughly equal.
    In the process of RNA-mediated DNA methylation, the bit specificity of DNA methylation is defined by two types of non-coding RNA: small interference RNA (siRNA) and stent RNA (scaffold RNA), which are produced by plant-specific RNA polymerases Pol IV and Pol V, respectively.
    therefore the researchers also analyzed the genome-wide levels of small interfering RNAs and stent RNAs at specific sites.
    they found that in pkl mutants, changes in DNA methylation levels at some sites were accompanied by changes in the same trend of small interference RNA levels and stent RNA levels.
    PKL also facilitates the positioning of multiple Pol V-adjusted nucleos, indicating that Pol V-related functions may be affected.
    by analyzing the correlation between mRNA transcription groups and genome-wide DNA methylation, the researchers found that in pkl mutants, although a certain number of transbens and gene transcription products rose along with a decrease in DNA methylation, most sites of DNA methylation changes were not sufficient to release transcriptional silence.
    therefore, the researchers envision that in the target area of RdDM, PKL can alter the chromosate environment through its nuclear micro-remodeling activity, thereby affecting the production of non-coding RNA and transcriptional silencing.
    this paper reveals the role of CHD family protein PKL in the RNA-mediated DNA methylation process and suggests that the RNA polymerase Pol IV/V can be tyrosed using the same chromosomal remodeling factors as Pol II.
    Yang Rong is the first author of this article and Zhang Wei is the author of the newsletter.
    work has been funded by the Chinese Academy of Sciences and other funds.
    .
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