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What are the common types of nucleic acid extractors? There are two classification methods for nucleic acid extractors, one is classified according to use, and the other is classified
according to different application reagents.
Classification and characteristics
of nucleic acid extractors.
(1) According to the use, it can be generally divided into two categories: one is the automatic liquid workstation, the automatic liquid workstation is a very powerful equipment, liquid dispensing, suction, etc.
are automatically completed, and even the expansion and detection of instruments and equipment can be integrated to realize the full automation
of specimen extraction, amplification and detection.
Nucleic acid extraction is only an application of its function, not very suitable for conventional laboratory nucleic acid extraction, generally used in a single type of specimen and a very large amount of specimens extracted at a time (at least 96, generally hundreds) experimental needs
.
The establishment of the platform of the automatic workstation and the operation of the platform require relatively large funds
.
The other type is small automation instruments, small automation instruments are through the special design of the operating structure to achieve the purpose of automatic extraction of nucleic acids, can be applied in any laboratory, in recent years there are many models on the market
.
(2) According to the different reagents applied, it is divided into two categories: one is the instrument that applies the magnetic bead reagent, and the other is the instrument
that applies the spin column reagent.
The automatic nucleic acid extractor using the magnetic bead method is easy to operate and automate, and is the mainstream on the market at present, such as QAGEN, Lab-Aid 820 and other instruments
。 What are the advantages and characteristics of nucleic acid extractors compared to traditional manual operations? Nucleic acid extractors generally have the following characteristics: (1) no cross-contamination: the instrument components are not in contact with biological samples; (2) Automation system: Each time the extraction work, the operator only needs to do a good job of installation, arrange the well plate (provide the reagents required for extraction).
The whole process of extraction does not require human help, and the instrument completes it automatically; (3) Extraction control: During the extraction process, if the operator needs it, the system allows you to control
the extraction process.