Clean standards and operating procedures of sterile rooms for microbiological testing

The sterile room (clean room) is the core of the microbiological testing room
.

(1) Clean room cleanliness standards

The cleanliness of the aseptic operation room should reach class 10,000, the negative cleanliness degree of ultra-clean work should reach class 100, the indoor temperature should be maintained at 18 ~ 27 ℃, and the humidity should be maintained at 30% ~ 70%
.

Table 4-3 Clean room (clean room) air cleanliness level

Note: 1.

2.
The minimum wind speed of the cross-section of the 0.
8m high working area of ​​the 100-class clean room (zone): vertical unidirectional flow 0.
25m/s, horizontal unidirectional flow 0.
35m/s
.

(2) Clean room operating procedures

(1) The sterile room should be kept clean, and it is strictly forbidden to pile up debris to prevent contamination
.

(2) The sterile room should be sterilized and cleaned with a suitable disinfectant regularly to ensure that the cleanliness meets the requirements
.

(3) Before entering the sterile room, the staff must wash their hands with soap or disinfectant to disinfect, and then change special work clothes, shoes, hats, masks and gloves in the buffer room
.

(4) Before using the sterile room, all items should be placed in the operating part (except the objects to be inspected), and then turn on the ultraviolet lamp to sterilize for more than 30 minutes, and at the same time turn on the ultra-clean workbench to blow air
.

(5) All items with live bacteria must be autoclaved before being cleaned or discarded
.

(6) Wipe the workbench, doors, windows, tables and chairs and the ground with a 3% carbolic acid aqueous solution every 2 to 3 weeks
.

(7) The number of colonies in the sterile room should be checked monthly
.