Clin Chem: A comprehensive drug resistance analysis of mycobacterium tuberculosis cultures using two commercial sequencing platforms.

!---- Because of the complexity of mycobacterium tuberculosis resistance, rapid and comprehensive drug sensitivity tests are required to guide patients in treatmentresearch and development of targeted sequencing workflows based on Illumina MiSeq and Nanopore MinION to predict the resistance of mycobacterium tuberculosis to 12 antibioticsmultiple PCR strains of 163m tuberculosis collected from Hong Kong and Ethiopia were, and 19 drug-resistant gene regions were amplifiedthen bar-coded and sequenced 24 and 12 samples on MiSeq and MinION, respectivelya web-based bioinformatics process: The BacterioChek-TB is used to turn raw data sets into reports that benefit cliniciansresults showed that the average reading depth of samples that were successfully sequenced by both platforms was 1127 and 1649 times, respectivelyif you exclude the allele frequency of the allele reported by MinION, the mIseq and MinION variant signals can reach 100% consistentThe average clinical sensitivity of the two workflows was 94.8% and clinical specificity was 98.0% compared to pDSTMiSeq and MinION workflows have 38 and 15 hours of turnaround time, respectively, providing treatment guidance 17-18 days ahead of pDST, respectivelythe higher cost per sample on the MinION platform ($71.56) and the MiSeq platform ($67.83) because of the different batch capabilitiesstudies have demonstrated the interchangeability of the MiSeq and MinION platforms, producing accurate and operational results for the treatment of tuberculosis.