Clin Gastroenterology H: Persistent diarrhea in patients with Crohn's disease with mucous membrane healing is associated with reduced diversity and increased malnutrition in the gut microbiome

In patients with inflammatory bowel disease (IBD), symptoms are not always directly related to the severity of mucous membrane inflammation under the endoscope, and the progression of the disease can be sustained after the mucous membrane heals.
, given that indigestion and diarrhea are the main characteristics of IBD, the researchers sought to prove a link between changes in tissue-related microbiomes and disease progression.
study looked at whether the symptoms of successful treatment of IBD patients were related to the composition of the gut microbiome.
researchers analyzed 590 tissue biopsy specimens from 215 IBD patients and 48 healthy individuals and obtained mucous membrane biopsy specimens from two colon sites (uplifted and rectal B colon) and the end of the rectum, as well as clinical data.
the bacterial DNA from biopsy specimens and sequenced the differences through the V4 region of 16s RNA.
biopsy specimens in patients with Crohn's disease (CD) reached mucosal healing (Mayo rated 0-1 or segment endoscopic severity CD score was 0-5) with less biodiversity than ulcerative colitis (UC) or unclassified patients with biopsy specimens IBD(IBD-U).
endoscopy showed no significant difference between the diversity of tissue-related microbiomes and the control group after an improvement in mucous membrane inflammation in UC or IBD-U patients.
compared to colon biopsy specimens in the control group, the micro-biodiversity of colon biopsy specimens in CD patients before and after rehabilitation was lower (P<.002>150; P s.03) and persistent diarrhea was associated with decreased micro-biodiversity (P s 0.01).
the microbial malnutrition index, persistent diarrhoea was found to be associated with trends in malnutrition (P - .059).
in UC or IBD-U patients with moderate to severe inflammation, the increased severity of diarrhea was associated with a decrease in microbiotics (P -.03).
In an analysis of biopsy specimens from IBD and control patients, the researchers found that despite endoscopy showing signs of improvement or remission, the α diversity of tissue-related gut microbiomes in CD patients was still lower than that of controls.
suggest that microbial group composition may be associated with persistent diarrhea.