echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > Cloning Long Polymerase Chain Reaction Products

    Cloning Long Polymerase Chain Reaction Products

    • Last Update: 2020-11-24
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

    A variety of methods have been developed and modified to facilitate the cloning of polymerase chain reaction (
    PCR
    ) products directly into plasmid. However, there are limitations in practice, including the low frequency of correct clones, the expense of commercially available cloning systems, and the need to add sequence or other modifications to primers to enable cloning. The more recent development of long and accurate PCR (LA-PCR) offers the ability to isolate much longer products. Using this technique, the vast majority of full-length eucaryotic mRNA transcripts can be easily amplified by reverse transcription and LA-PCR. In addition, since the coding region for the majority of mammalian genes is contained within approx 20 kilobases (kb) or less of genomic
    DNA
    , this technology can be used to rapidly obtain genomic sequence corresponding to known
    cDNA
    s.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.