Cloning: Study of the molecular mechanism of one-way hybridization of corn.

Affinity is the biological basis for the evolution and formation of species, which determines and balances the diversity and stability of plant populations on Earth.
analysis of the molecular mechanism sourcing and controlling the affability between and within plantspecies has been a hot topic in botany research.
corn is a typical hetero-flower pollination crop, which is usually normally strong for both self-breeding and hybridization.
however, some corn materials in nature do not accept foreign pollen and insemination is strong, a phenomenon known as one-way hybridism (Unilateral Cross-Incompatibility, UCI).
because UCI affects the direction of transmission of pollen dispensers, the gene that controls the phenomenon is called Gametophyte Factor (Ga).
the first corn one-way hybrid non-affinity site (Ga1) was first discovered by Correns in 1902, is one of the most thorough sites of one-way hybridaffility, and is widely used in the breeding and production of burst corn.
several subsequent sites that control the affluin affinity of corn hybridization have been discovered, but no genes have been cloned.
more than a hundred years, the molecular mechanism of corn UCI has been a mystery.
, in cooperation with Zhou Weihua Research Group and Xue Yongxuan Research Group, the Chen hua group of the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences made a breakthrough in the field of corn one-way hybridization and gene research, successfully cloning the gene ZmGa1P, which controls the phenomenon of one-way hybridization of corn, and exploring its mechanism for the first time.
the study team of Chenlisting list has been studying the phenomenon of corn hybridaffration in Ga1 site control since 2007, and proposed a two-factor genetic control model of Ga1 site.
the study used 22,000 single strains of homogenous group to genetically locate zmGa1P, the male control gene in Ga1, combined with 1299 manotle and genotype data from the manotle and genotype of the manotle, and used the 600K SNP chip to perform genome-wide correlation analysis to determine the candidate genes.
the resulting clone of ZmGa1P by filtering and sequencing the BAC library of the Ga1-S self-intersecting system built by Xue Yongxuan's research team.
the validation of genetically modified functions confirmed the correctness of the cloned gene.
ZmGa1P encodes a pectoral lyse expression in Ga1-S and Ga1-M corn self-actonicant medicine (Pectin methylesterase, PME).
Zhou Weihua research team through a large number of biochemical and cytological research, found that ZmGa1P is located at the top of the pollen tube, with another pollen tube specific expression of THE PME protein interaction, together to maintain the pollen tube normal level of methyl esteration modification, to ensure the normal elongation of the pollen tube in Ga1-S-type filament, and eventually fertilized strong.
the successful cloning of the ZmGa1P gene created the conditions for the reproductive isolation of non-isolated hybrid strains of maize, special corn and common corn, and genetically modified and non-GMO maize.
through years of breeding practice, the Chenhua list research group has used this site to breed the first hybrid non-affinity corn in China, to achieve the phenomenon of non-affinity of corn non-isolation application.
theory, unlike the previously discovered plant interspecies and intraspecies affinity-mediated degradation pathways, the study first found that non-affinity in corn is controlled by the PME complex that regulates the growth of pollen tubes, and the mechanism may also be prevalent in single-leaf plants.
, therefore, this achievement has important theoretical innovation and application prospects.
published on September 10 in the journal Nature Communications (DOI: 10.1038/s41467-018-06139-8). Zhang Zhaogui, a ph.d. student at the chenhualist research group in
, and Zhang Baocai, an associate researcher in Zhou Weihua's research group, co-authored the paper.
the research was supported by the GM special project, the national key research and development plan, the National Natural Science Foundation of China, and the Youth Innovation Promotion Association of the Chinese Academy of Sciences.
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