Close to complete 90S UC structure model construction.

RNA is a large molecular machine made up of RNA and a large number of proteins responsible for the synthesis of proteins from all living things on Earth.
in the uerrogen, the assembly of the ucose is a very complex process.
the kernels need to be temporarily combined with a large number of assembly factors in the process of maturation, forming a series of UC presupposion complexes.
small sub-base kerucleosomes form two main intermediates during assembly: the early 90S and the late pre-40S presupposes.
90S presupposes are a huge complex that contains about 50 non-RNA proteins and U3 snoRNAs, in addition to RNA and protein components, with a molecular weight of up to 5 million Daltons.
The Yek Poor Laboratory of the Institute of Biophysics of the Chinese Academy of Sciences obtained three electron density maps of germinated yeast 90S ucose presupposes using frozen electron mirrors and single-particle reconstruction techniques, the best of which achieved an overall resolution of 4.5 E.
using known crystal structures, head-to-head modeling, and chemical cross-linked mass spectrometry data to build a near-complete 90S structural model.
structure of the 90S shows that the small sub-base folding of the newborn cysome forms multiple separate substructages and binds to a large number of assembly factors.
5' interval region of RNA, U3 snoRNA, and a large number of assembly factors to form a huge base to support the structure of the newborn RNA.
structure also reveals novel ways in which U3 snoRNA and RNA, a presupposor of rna, are combined.
structure is a milestone in understanding the early assembly principles and the function of assembly factors of the small sub-base of the cystic body.
paper on the work, Molecular architecture of the 90S small subunit pre-ribosome, was published online February 28 in eLife magazine.
Ye Ke poor is the paper's communication author, Sun Qi, Zhu Xing, Qi Jia and An Weidong are co-first authors.
and Tan Dan, as well as a number of researchers from the Ye Ke Poor Task Force, also contributed to the study.
research equipment and technical support for the study from the Center for Bioimaging of the Chinese Academy of Sciences.
the study was supported by the National Natural Science Foundation of China, the Chinese Academy of Sciences Strategic Pilot Science and Technology Special (Category B), the Ministry of Science and Technology and the Beijing Municipal Government.
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