Common Compositions of Buffers and Buffers Commonly Used in Biologics

In this article, we will introduce the common composition of buffers and common buffers in the field of biological preparations! Common composition of buffer solutions Acids commonly used as buffer solutions A solution composed of weak acids and their conjugated salts has a buffering effect
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Common buffer systems are: (1) Weak acid and its salt (such as HAc—NaAc) (2) Weak base and its salt (NH3·H2O—NH4Cl) (3) Acid salt of polybasic weak acid and its corresponding secondary It is composed of an aqueous solution of grade salt (such as NaH2PO4—Na2HPO4)
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 Commonly used buffers in the field of biological preparations The commonly used buffers in the field of biological preparations mainly include phosphoric acid, citric acid, carbonic acid, acetic acid, bartonic acid, Tris (tris (tris(hydroxymethylaminomethane)) and other systems
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In biochemical experiments or research work, the buffer system should be carefully selected, because sometimes the factor affecting the experimental results is not the pH value of the buffer, but a certain ion in the buffer
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Buffers such as borates, citrates, phosphates, and trimethylolmethane can all produce unwanted chemical reactions
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(1) Borates: Borates form double salts with many compounds, such as sucrose
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(2) Citrate: Citrate ions are easily combined with calcium, so they cannot be used in the presence of calcium ions
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(3) Phosphate: In some experiments, it is an inhibitor of enzymes or even a metabolite, and heavy metals are easily precipitated out of solution in the form of phosphates
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And it has little buffering capacity above pH 7.
5
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