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it turns out that in today's rapid development of science, no matter what field of research, in addition to a good theoretical basis, more importantly, rely on advanced technology and > excellentand a good research environment. In addition to conventional instruments in general biology laboratories, a standard"> molecular biology" laboratory also has special-purpose instruments, which are generally more sophisticated and expensive. Here's how to use these instruments and what to do.
1, frozen> centrifuges
cryogenic separation technology is an essential means of molecular biology research. the separation of fragments ofgene, the precipitation and recovery of enzyme proteins, and the separation and preparation of other biological samples are inseparable from cryogenic centrifugation technology, so cryogenic cryo-freezing centrifuges have become an important instrument in molecular biology research.
in China, there are several manufacturers of frozen centrifuges, the laboratory's high-speed frozen centrifuges for the GL-20G-II(Shanghai Anting), floor-to-ceiling. Comes with angled turns: 6×50ml, 12×10ml and 12×1.5ml. Extreme speed 20000rpm.
1. Installation and commissioning
< text-right: left; > centrifuges should be placed on a level of solid ground, should be at least 10 cm away and have good ventilation environment, the surrounding air should be neutral, and non-conductive dust, flammable gases and corrosive gases, the ambient temperature should be less than 0 to 30 degrees C, relative humidity 80%. Before the trial turn should first open the door, with the hand to move the shaft, light and flexible, no abnormal phenomenon to use the turn.
roor accurately in place after turning on the power switch, and then press and hold the door switch by hand, then press the operating key, immediately stop after turning, and observe the steering of the shaft, if counterclockwise rotation is correct, the machine can be put into use.
2. operating program
(1) plugged in the power supply, standby light on, turn on the power switch, speed control and time system digital tube display flashing number for the machine operating speed factory setting, the temperature control system digital tube shows the temperature of the centrifugal cavity at this time.
< p style is "text-align: left;" >(2) sets the operating parameters of the machine, such as operating temperature, running time, operating speed, etc.(3) places the pre-balanced sample on the turnthead sample holder and closes the lid.
(4) press the control panel's operating key and the centrifuge starts running. During a pre-set acceleration time, its speed rises to a pre-set value.
(5) in a pre-set operating time (excluding deceleration time), the centrifuge begins to decelerate, and its speed drops to zero within a pre-set deceleration time.
(6) presses the stop key on the control panel, the digital tube displays the dedT, and after a few seconds the flashing speed value is displayed, when the machine is ready for the next operation.
3. Precautions
(1) centrifuges should always be in a horizontal position, the voltage of the external power system should match, and require a good ground wire, the machine is not in use, to unplug the power supply.
(2) before powering on should check that the turntable installation is secure and that there is no foreign material falling into the cavity.
(3) sample should be pre-balanced, centrifuge cylinder and sample should be balanced at the same time when centrifuging with centrifuges.
(4) Volatile or corrosive liquid centrifugation, use a coveredcentrifuge tube and ensure that the liquid does not leak to avoid corrosion of the cavity or cause accidents.
(5) Do not change the operating parameters of the machine at will, except for operating temperature, operating speed and operating time, so as not to affect the performance of the machine. The speed setting must not exceed the maximum speed to ensure the safe operation of the machine.
(6) if the use of 0.00 or other numbers, the machine does not run, should be powered off, 10 seconds after re-starting, until the set speed display, and then press the operating key, the machine will operate as usual.
(7) do not open the door while the machine is running or the roor is not stable in order to avoid an accident.
(8) should be used to keep a good record of usage every time it is completed and the performance of the machine should be overhauled on a regular basis.
II, electrophoresis instrument
electrophoresis technology is an indispensable and important means of molecular biology research. Electrophoresis is generally divided into free interface electrophoresis and district electric swimming categories, free interface electrophoresis does not need support, such as iso-speed electrophoresis, density gradient electrophoresis and microelectronic swimming, such electrophoresis is currently rarely used.
area electric phorescies need to use various types of substances as support, commonly used support items arefilter paper, acetic fiber film, non-gel support, gel support and silicone? DG thin layer, molecular biology experiments, the most commonly used isagarsugar gel electrophoresis.
can use electrophoresis to perform qualitative or quantitative analysis of different substances, or to analyze a certain mixture or extract a single component. Here is the DYY-12 computer sanhengdo electrophoresis instrument (Beijing 61 instrument factory) as an example to introduce its use.
1. Use method
(1) press the power switch, the display appears "Welcome to dyY-12 computer three constant multi-electrophoresis meter..." and so on, at the same time the system initialization, beeping 4 sounds, settings often set. The screen turns into a parameter setting state, see Figure 1:
U: 0V U-100V STD I:0mA I s 50mA s P: 0W P - 50W - T:00:00 T - 01:00 - . |
where: top-left U: I: P: T: actual value for electrophoresis; Mode: STD (standard); TIME (time); VH (volt time); STEP (step-by-step)
(2) set up the work program. Enter a new work program with the keyboard. For example, the operating voltage is required to be U-1000V, the current I is limited to 200mA, the power W is limited to 100W, the time T is 3 hours and 20 minutes, and the output is automatically turned off at the time. Here's how to do this:
(1) press the Mode key to change the operating mode from standard (STD) to timed (TIME
mode. With each press of the mode key, the way it works changes in the following order: STD®TIME®VH® STEP®STD.
(2) first sets the voltage U, presses the "Select" key, reverses it, and then enters the number key to set the value of the parameter. By the number 1000, the voltage is set.
< p style is "text-align: left;" >(3) sets current I, presses the "Select" key, reverses I, and then enters the number 200. < p style is "text-align: left;" >(4) sets power P, presses the "Select" key, reverses P, and then enters the number 100. < p style is "text-align: left;" >(5) sets the time T, presses the "Select" key, reverses the T, and then enters the number 320. If the input is incorrect, you can press the Clear key and re-enter it.(6) After confirming that the parameters are correct, press the "Start" key to start the electrophoresis output program. Show Start in the display status bar! And beep 4 sound, remind the operator electrophoresis meter will output high voltage, pay attention to safety. The output voltage is then gradually added to the set value. Run is also displayed in the status bar with two flashing high-voltage symbols indicating that the port already has a voltage output. At the bottom of the status bar, the actual working time (accurate to seconds) is displayed.
(7) Automatically stores the setting value of this time into the "MO" storage unit each time the output is started. Later, when you need to call, you can press the "Read" key, then press the "0" key, press the "OK" key, you can take out the last set of work program to execute.
(8) electrophoresis ends with the instrument displaying:END" and a continuous beep alert. Press either key to stop the
.
