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    Home > Biochemistry News > Biotechnology News > Common reagent preparation small common sense -- you know?

    Common reagent preparation small common sense -- you know?

    • Last Update: 2020-11-01
    • Source: Internet
    • Author: User
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    1, which concentration of alcohol disinfection effect is best?
    75% alcohol is the most bactericidal, it can make
    protein
    dehydration and denaturation, killing bacteria in 3 to 5 minutes. Therefore, it is used for disinfection and corrosion protection, suitable for skin and equipment, plastic products, etc. disinfection. High concentrations of alcohol (95 to 100%) can cause the bacteria surface protein solidification, the formation of a protective layer, so that alcohol molecules are not easily penetrated, so the ability to sterilization is weak.
    the
    of reagents
    2, reagents?
    according to the purity of
    chemical reagents
    , according to the amount of impurity content can be divided into four levels:
    -level reagents for high-quality pure reagents, usually with G. R indicates that
    secondary reagent is an analytical pure reagent, usually with A. R indicates that
    -level reagents are pure chemical reagents, usually with C. R indicates that
    -level reagents are experimental or industrial reagents, usually with L. R indicates.
    , according to special work purposes, there are some special purity standards, such as
    spectral
    pure, fluorescent pure, semiconductor pure and so on. Use should be in accordance with different experimental requirements, the selection of different specifications of reagents.
    do you know about the dissolution of 3, solid reagents?
    solid particles should be crushed before dissolving the larger solid particles. The crushing of solids is carried out
    the
    and drying of the material. The amount of solids in the study should not exceed one-third of the total capacity of the research. Dissolve solids, often stirring,
    heating
    and other methods to speed up the dissolution speed.
    the mixing liquid should be held with a mixing stick and turn the wrist, do not turn too fast, do not let the mixing rod hit the wall of the device. When stirring the liquid in the test tube, the mixing rod can be turned or gently stirred up and down, but do not use too much force to avoid breaking the test tube. The method of oscillating test tube can also be used instead of stirring.
    method of heating to accelerate the dissolution of solids is the same as when heating liquids, i.e. there are generally direct heating methods and water bath heating methods. Depending on the stability of the heated substance, different heating methods are used. Also take care to cover the container with surface dishes to prevent the liquid from evaporating.
    4, are you paying attention to this?
    the
    balance of
    weighing reagents should be kept clean and dry to avoid moisture and corrosive gas erosion. During weighing operations, the reagents weighed should be placed on weighing paper or other vessels and not weighed directly in the dish.
    the preparation of strong acids, strong alkali solutions or contact with toxic drugs, should be carried out in strict accordance with operating procedures. If diluting sulfuric acid, should be careful to slowly pour thick sulfuric acid into the water, do not pour water into the sulfuric acid.
    the maximum amount of the drug solution injected by an animal determines the concentration of the drug solution in the preparation of the drug solution. If the intravenous fluid capacity is too large, it can affect the normal function of its circulatory system. Therefore, intravenous capacity is best below 1/100 of the weight, intravenous (subsulvert, muscle and abdominal cavity) injection capacity is best below 1/40 of the weight, therefore, according to the weight of the animal, the appropriate concentration of the drug solution.
    the preparation of pharmaceutical solutions, the stability of the environment in experimental animals and the normal function of animal ionosomes or
    tissues
    under experimental conditions should be taken into account. An equilibrium with blood and body fluids, as well as isopH and ion balance, should be achieved, and physiological saline preparation should be used (0.9% NaCl for warm-blooded animals and 0.6% NaCl for cold-blooded animals).
    ionized organ perfusion fluid or nutrient solution due to the large amount of use, should be strictly calculated permeable pressure, pH and various ion concentrations, and sometimes also take into account nutrients such as glucose content, according to the different animal organs and the use of different physiological solutions.
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