Comparison of microsatellite polymorphism differences between transcription groups and genome screening.

High-throughput sequencing has been increasingly used in biological research.
these massive amounts of data contain a variety of molecular markers that can be used for evolutionary and conservation research.
, microsatellite molecular markers are widely used in population and genetic research because of their high polymorphism and easy amplification.
Xia Yun, a member of the Chengdu Institute of Biological Research of the Chinese Academy of Sciences, discussed the effects of different sources, different splicing methods and sequencing depth on microsatellite screening results against the difficulty of interspecies proliferation of amphibious microsatellites, while high-throughput sequencing is relatively convenient.
provides a comprehensive assessment of the use of high-throughput data to filter microsatellite markers.
study found that the number of microsatellite screeningincreases with the increase in data volume and read length.
data stitching strategy will significantly affect the screening results of microsatellites, that is, the larger the K value, the fewer microsatellites produced, but the number of repetitions of these sites, suggesting a higher polymorphism.
, the microsatellite sites obtained by the transcription group showed lower polymorphismrelativerelatives relative to genomic sites but were more easily related to functional sites.
recent findings were published in the international journal Genomics BMC Genomics on microsatellite development from the skimming and risome dating: comparison of the strategies and lessons from the world frog species.
Source: Chengdu Institute of Biology.