Comparison of new crown nucleic acid extraction efficiency and discussion on influencing factors

Author: Jiaozhou Hospital, Dongfang Hospital Affiliated to Tongji University---Wang Xiaoyan, Teng Yiming

Author: Jiaozhou Hospital, Dongfang Hospital, Tongji University---Wang Xiaoyan, Teng Yiming

 

Real-time fluorescent quantitative PCR detection technology has the advantages of strong specificity, high sensitivity, and good stability, but there are many factors that affect its detection effect.

[1] [2]

Next, we compare the extraction efficiency of the two extraction systems in our laboratory through the CT value of RT-PCR, and discuss the influencing factors

 

Principle of nucleic acid extraction by magnetic bead method

CT mean value of laboratory detection system and RT-PCR

Comparison result

From the CT mean values ​​in the table above, it can be seen that the Zhongyuan extraction system has higher extraction efficiency for the ORF1ab target gene fragment, and the Zhijiang extraction system has higher extraction efficiency for the N target gene fragment

Analysis of Results and Discussion on Influencing Factors

On March 31, 2020, the State Drug Administration issued the Pharmaceutical Industry Standard of the People's Republic of China (YY/T 1717--2020)--Nucleic Acid Extraction Kit (Magnetic Bead Method), which will be implemented on April 1, 2021

Part 5.

The third edition of "Clinical Molecular Biology Test" pointed out that quality management in analysis includes nucleic acid extraction, amplification and product analysis

1.

1.

2.

3.

[3]

 

references

references

[1] Gui Fei, Yang Weiwei, Yu Liping, Comparison of the extraction efficiency of mouse hepatitis virus nucleic acid from fecal samples by six nucleic acid extraction kits [J] Laboratory Animal Science, 2019, 36(1): 31-34

[1] Gui Fei, Yang Weiwei, Yu Liping, Comparison of the extraction efficiency of mouse hepatitis virus nucleic acid from fecal samples by six nucleic acid extraction kits [J] Laboratory Animal Science, 2019, 36(1): 31-34

[2] Dong Hao, Han Tao, Bi Yiming, Yuan Lin, etc.

[2] Dong Hao, Han Tao, Bi Yiming, Yuan Lin, etc.

[3] Tong Jianhua, Lou Jiatao, Liu Xiangfan, "Clinical Test Ten Thousand Why Molecular Biology Test Volume", the first edition of May 2018

This article was originally created by the Voice of Inspection, please indicate the source for reprinting

This article was originally created by the Voice of Inspection, please indicate the source for reprinting

Editor: Green Desires Editor: Green Desires Editors: Green Desires

Reviewer: Chen Chen

Review: Chenchen Review: Chenchen Review: Chenchen

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