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A team of engineers at the University of Delaware has developed a method that uses CRISPR/Cas9 technology to trigger a series of activities in cells, a phenomenon known as conditional gene regulation.
their approach is described in the Nature Chemical Biology journal, which provides crispR with a new feature, one of today's most-watched technologies.
using CRISPR technology for gene editing has been described as "one of the biggest scientific stories of the decade" because of its use in medicine, agriculture and so on.
CRISPR allows scientists to accurately target and edit DNA in living cells, which can help them correct abnormalities that lead to genetic disorders.
the first clinical trials of humans are being conducted in China.
, however, scientists have not figured out how to program their CRISPR system to target DNA while integrating information from the cells they are studying.
in UD, Professor Wilfred Chen of The Chemical Engineering, and graduate student Ka-Hei Siu designed the structure - called gated gated gRNA ( thgRNA ) - for targeted gene regulation in E. coli.
traditionally, in CRISPR/Cas9 genome editing, scientists used single-stranded rnanucleonucleic acid (RNA) to direct Cas9 enzymes to the DNA they wanted to target.
, Instead, Chen and Siu installed hairpin-like structures that prevent some RNAs from identifying DNA.
only a small fraction, called a foothold, exposed and capable of binding to other RNAs.
then Chen and Siu use RNA from within cells as a trigger to open up their blocking mechanisms and activate the Cas9 protein so that it binds and regulates DNA. "The key thing
is that we want to use some local cell information," Chen said. "We wanted to use this natural cellular response as a way to regulate the crispR/Cas9 protein function and basically developed a controlled mechanism so that we could regulate cell function accordingly,"
.
" Chen says the technology provides a versatile "plug-and-play" design that can be used to induce gene editing and regulation in a variety of systems.
"Looking forward, the idea is to theoretically use any type of cell messenger RNA on paper as an activation or deactivation device," he said.
", "As you can imagine, we can activate something based on whether the cells are growing on glucose or if they are deficient in phosphate or exposed to high temperatures or low pH conditions."
" Source: Thundernet Space.