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Construction of Infectious Clones of Double-Stranded DNA Viruses of Plants Using Citrus Yellow Mosaic Virus as an Example

 Last Update: 2020-11-06
 Source: Internet
 Author: User

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virus length

what genomic dna

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Double-stranded
DNA
(dsDNA) viruses of plants are believed to be plant pararetroviruses. Their genome is replicated by reverse transcription of a larger than unit-length terminally redundant RNA transcript of the viral genomic DNA using the virus-encoded replicase. In order to produce a cloned, infectious viral genome, the clone must be constructed in a binary vector and be longer than the full, unit-length viral genome. The clone can then be transferred by
Agrobacterium
-assisted inoculation into a suitable host plant to induce virus infection.

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