Correct use of Class II biological clean safety cabinet

Biosafety cabinet is necessary for microbiological laboratory, especially for those occasions where operators need to take protective measures Such as medical, pharmaceutical, scientific research and other bacterial culture to provide both sterile and dust-free working safety environment Features: 1 Air curtain type isolation design, to prevent cross pollution inside and outside, 70% air flow outside, 30% internal circulation, negative pressure vertical laminar flow 2 Move the glass door up and down, which can be positioned at will, easy to operate, and can be completely closed for sterilization, positioning height limit alarm prompt 3 The power output socket in the working area is equipped with waterproof socket and blowdown interface to provide great convenience for the operator 4 The exhaust area is equipped with special filter to control the emission pollution 5 The working environment is made of 304 stainless steel, which is smooth, seamless and free of dead angle It can be easily and thoroughly disinfected, and can prevent the corrosion of corrosion agents and disinfectants 6 Led liquid crystal panel is used for control 7 Equipped with filter failure alarm function 8 Air flow outflow alarm in the working area Technical parameter model bhc-13000iia / B2 cleanliness class 100 @ ≥ 0.5 μ m (US Federation 209E) colony number Noise ≤ 62db (a) average wind speed (0.38 + - 0.025m/s) inside the door when < 0.5 plate / time (90mm culture plate) Average wind speed 0.26 + -0.025m/s in the middle, average wind speed 0.27 + -0.025m/s in the inner ＜ 0.5um (x, y, z direction) illumination ≥ 300lx front suction wind speed 0.35 + - 0.025m/s Air density < = 10-6m / S (under 500pa pressure) power supply AC, single-phase 220V / 50Hz Zui high power consumption 600W Weight: 350kg, working dimension: 1000 * 700 * 680mm, overall dimension 1200 * 780 * 1950mm filter specification and quantity 995 × 640 × 38 × 1mm fluorescent lamp / UV lamp specification and quantity 16W * 2 / 15W * 2 Model bhc-13000iia / B3 class 100 cleanliness @ ≥ 0.5 μ m (209E) colony count Noise ≤ 62db (a) average wind speed (0.38 + - 0.025m/s) inside the door when < 0.5 plate / time (90mm culture plate) Average wind speed 0.26 + -0.025m/s in the middle, average wind speed 0.27 + -0.025m/s in the inner ＜ 5um (x, y, z direction) illuminance ≥ 300lx air density before suction wind speed 0.55 + -0.025m/s < 10-6m / S (under 500pa) power supply AC, single-phase 220V / 50Hz Zui high power consumption 600W Weight: 350kg, working dimension: 1340 * 650 * 600mm, overall dimension: 154 * 800 * 1950mm Filter specification and quantity 1335 × 600 × 38 × 1mm fluorescent / UV lamp specification and quantity 16W * 2 / 15W * 2 Model bsc-1000iia2 biological clean safety cabinet bsc-1300iia2 biological clean safety cabinet bsc-1600iia2 biological clean safety cabinet Working area dimension (w × D × h) 1000 × 650 × 580 1300 × 650 × 580 1600 × 650 × 580 device boundary dimension (w × D × h) 1200 × 750 × 1950 1500 × 750 × 1950 1800 × 750 × 1950 purification efficiency class 100 (US federal standard 209E) weight 150kg 200kg 250kg average wind speed door inner side: 0.38 ± 0.025m/s inner side: 0.27 ± 0.025m/s middle side: 0.26 ± 0.025m/s Front suction wind speed 0.35 ± 0.025m/s power consumption 0.8kw average colony count ≤ 0.5 dishes · H Noise ≤ 62db, vibration half peak ≤ 3 μ m (x · y · z), power supply 220V50Hz Filter size (L × w × h) mm 955 × 545 × 50 1295 × 545 × 50 1595 × 545 × 50 lamp / germicidal lamp Precautions: 1 Slow moving principle: in order to avoid affecting the indoor air path state, the operation should move as smoothly as possible 2 Principle of parallel placement of articles: in order to avoid cross contamination between articles, articles placed in the cabinet should be placed horizontally as far as possible to avoid cross contamination in the process of return air At the same time, avoid blocking the back return air grille to affect the normal air path 3 Principle of avoiding vibration: try to avoid the use of vibration instruments (such as centrifuge, vortex oscillator, etc.) in the cabinet, because vibration will shake off the particles accumulated on the filter membrane, which will reduce the cleanliness of the operation room, and at the same time, if the front operation surface fails to balance, it will cause pollution of the safety cabinet to the operator 4 Moving principle in different sample cabinets: when two or more items need to be moved in the cabinet, the principle of moving low polluting items to high polluting items must be followed to avoid large-area pollution of high polluting items in the moving process The experimental operation on the worktable should be carried out in the direction from clean area, semi polluted area to polluted area to prevent cross contamination Equipment that can produce aerosol (such as mixer, centrifuge, etc.) shall be placed close to the back of the safety cabinet, and articles shall be placed as far back as possible, but the airway shall not be blocked, so as not to interfere with the normal flow of air flow 5 Use principle of open fire: try not to use open fire in the cabinet! Because the fine particle impurities produced in the use of open fire will be brought into the membrane area, these high-temperature impurities will damage the membrane When it is unavoidable to use it, the Bunsen burner with low flame should be used 6 The test can be conducted on the towel full of disinfectant to absorb the liquid drop that may spill After the completion of the experiment, all the equipment and articles taken out from the safety cabinet should be strictly disinfected Since the biosafety cabinet continues to turn on the fan after the experiment, generally Zui will turn on the fan for 15-30 minutes less and turn on the UV light for disinfection at the same time,