Human Cotinine enzyme-linked immunoassay (ELISA)

reagents are for research purposes only<" "">Purpose: This kit is used to determine the content of humanserum, plasma and related liquid samples of cotinine .

experimental principle:

this kit applies a doubleantibody clamping method to determine the level of human-to-tinting< "">" in the specimen. With purified people can< ">" (Cotinine)antibody packageMicroplates , made into solid-phase antibodies, and added cantinstyle" to the micro-holes that are single-resistant to the package < >, and then with"">HRPlabeled tidytin (Cotinine) < "" > antibody, the formation of antibodies - <" > < a href""" > antigen - ase-labeled antibody complex, after thorough washing of the substrate TMB color. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The shade of color is positively related to the < of the > in the sample "" Measure the absorbent at 450nm wavelength" ( OD> " and the concentration of human can be used in the sample can be measured by the standard curve "" concentration.

" kit :

1 Part