CRISPR/Cas9 directed epigenetic editing reveals multiple transcriptional regulation and functional effects

"Oncotarget" published "Diverse transcriptional regulation and functional effects revealed by CRISPR/Cas9-directed epigenetic editing", reporting that the full function of this process is essential for the determination of tissue homeostasis and cell fate

It is worth noting that this modification resulted in the opposite expression profile of target genes in different cancer cell models, and affected the expression of mesenchymal genes CDH1, VIM1, TGFB1 and apoptosis marker BCL2

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Examples of various genes having opposite effects, for example, DNA methylation may be one of the driving factors of these opposite effects

In this study, the authors focused on insulin-like growth factor binding protein 2, which is a recently discovered multitasking gene that is regulated by DNA methylation and has also been reported as a tumor-promoting and suppressor gene

DNA methylation involves an important mechanism involving transcriptome regulation based on the CpG dinucleotide occupying the 5'-methylcytosine chemical group

The Vizoso/van Rheenen research team concluded in their Oncotarget research output, “Our research emphasizes the exploration of epigenetic editing in different tumor environments by revealing the important consequences of epigenetic editing on transcriptome regulation and tumor cell behavior.

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Article title

Diverse transcriptional regulation and functional effects revealed by CRISPR/Cas9-directed epigenetic editing