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Compared with rapid diagnosis and genetic testing, the author values evaluating the accuracy of CRISPR-based gene editing techniques.
at present, the reason why the technology is used by the application of the limited problem, it is because it has a certain proportion of off-target problems in practical application.
therefore, spare no effort to improve editing accuracy, is to let CRISPR "gene shear" to the full potential of tool-based technology should be done.
a team at the Keck Institute at the University of California, Berkeley, and Claremont College combined gene editing with nanoelectronics to create a new type of handheld device that can detect specific genetic mutations in minutes.
researchers say the device, known as CRISPR-Chip, is quick and easy and can be used to quickly diagnose genetic diseases or evaluate the accuracy of gene editing techniques.
the CRISPR-Cas9 technology that has emerged in recent years, known for its precision, provides researchers with unprecedented gene editing.
to take advantage of CRISPR's genetic targeting capabilities, the team connected a inactivated Cas9 protein variant, a Cas9 variant that can find a specific site in DNA but not cut it, attached to transistors made of graphene.
when this CRISPR complex finds a specific site in its targeted DNA, it binds to it and triggers graphene conductivity changes, changing the electrical properties of the transistor.
these changes can be detected by handheld devices developed by the team.
researchers point out that it is not difficult to detect a genetic mutation through DNA sequencing, and many hospitals or companies can provide such services.
but it's easier and faster to test for genetic mutations with CRISPR-Chip than most genetic tests, and can be done in a doctor's office or in a field work environment without having to send samples to the lab. when
, simply place the extracted DNA sample on the chip and search through CRISPR technology, graphene transistors can report search results in minutes.
researchers say the new device is a combination of nanoelectronics and modern biology that combines the precise properties of CRISPR technology with the ultrasensitivity of graphene transistors, which are both accurate and fast.
the device can be used to diagnose diseases quickly, to help doctors develop personalized treatment plans for patients through rapid genetic testing, or to monitor whether CRISPR is combined with specific DNA sequences to assess the accuracy of CRISPR-based gene editing techniques.
research published online in the journal Nature Biomedical Engineering.
Source: Science Daily.