Crosstalk between Toll like receptors and specific autophagic proteins may affect AMPK activation

Recently, a research report titled "TLR9 and Beclin 1 crosstalk regulations muscle activation in exercise" was published in the international journal Nature Scientists from Southwest Medical Center and other institutions revealed the total like receptor 9 (TLR9) and protein Beclin during exercise through research 1 Crosstalk regulates the molecular mechanism of AMPK activation in muscle Image source: lumen learning The activation of adenosine monophosphate activated protein kinase (AMPK) in skeletal muscle is coordinated with the systemic metabolic response caused by exercise During exercise, autophagy is up-regulated Autophagy is a lysosome degradation pathway that can maintain a stable and balanced environment in cells In skeletal muscle, Beclin The core autophagy protein of 1 is essential for AMPK activation In this study, the researchers revealed the innate immune sensing molecules TLR9 and Beclin 1 protein interaction plays a key role in the activation of AMPK in exercise-induced skeletal muscle; the researchers found that mice without TLR9 may also have defects in the activation of exercise-induced AMPK, and the location of GLUT4 glucose transporter in skeletal muscle plasma membrane also has some defects, but may not show defects in autophagy; TLR9 The interaction between BCL2 and Beclin 1 can be increased by energy pressure, i.e glucose starvation and endurance exercise, but decreased by BCL2 mutation, which can block the binding between BCL2 and Beclin 1 protein TLR9 can regulate the assembly of pi3kc3-c2 in skeletal muscle during exercise Pi3kc3-c2 is an endolysosomal phosphatidylinositol 3-kinase complex, which contains Beclin 1 and UVRAG molecules Knocking out Beclin 1 or UVRAG will inhibit the induction of AMPK activation in cells by glucose starvation In addition, TLR9 can induce AMPK activation, glucose intake and Beclin by stereo contraction The assembly of 1-uvrag complex will work in a muscle independent way In the end, the researchers said that the results revealed the key role of Toll like receptors in the activation of AMPK and glucose metabolism in skeletal muscle during exercise, and also found an unknown crosstalk mechanism between innate immune sensors and autophagy proteins Original source: