Cryopreservation of Fish Sperm
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Last Update: 2021-03-01
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Source: Internet
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Author: User
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This chapter provides detailed descriptions of the protocol used for fish sperm cryopreservation. The principles describedcan be applied to any species, but details are also given for individual fish species. Successful cryopreservation of fishspermatozoa depends on a range of factors including the collection of high quality sperm, equilibration conditions, choiceof cryoprotectant medium, cooling/thawing regimes, and conditions for fertilization. Even though some general rules can beapplied to any fish species, optimization of the protocol is needed for each individual species. Furthermore, because spermderived from marine and freshwater fish differs markedly, the described protocol is mainly focused on the cryopreservationof sperm for freshwater fish because this poses the greater technical challenge.
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