Cryopreservation of Human Embryonic Stem Cell Lines
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Last Update: 2021-01-02
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Source: Internet
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Author: User
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Two different approaches have been adopted for the cryopreservation of human embryonic stem cells (hESCs): vitrification andconventional slow cooling/rapid warming. The vitrification method described here is designed for hESCs that grow as discretecolonies on a feeder cell monolayer, and are subcultured by manual subdivision of the colonies into multicellular clumps.hESCs that are subcultured by enzymatic dissociation can more conveniently be cryopreserved by conventional slow cooling/rapidwarming methods. Although both methods are suitable for use in a research context, neither is suitable for cryopreservationof embryonic stem cells destined for clinical diagnostic or therapeutic uses without modification.
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