Cytokine Analysis by Immunoaffinity Capillary Electrophoresis
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Last Update: 2020-11-26
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Source: Internet
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Author: User
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Immunoaffinity capillary electrophoresis (ICE) is a powerful tool used to detect and quantify target proteins of interestin complex biological fluids. The target analyte is captured and bound to antibodies immobilized onto the wall of a capillary,labeled in situ with a fluorescent dye, eluted and detected online using laser-induced fluorescence following electrophoreticseparation. Here, we illustrate how to construct an immunoaffinity capillary and utilize it to run ICE in order to captureand quantify target cytokines and chemokines from a clinical sample.
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