Dali rotonite enzymes are involved in a new mechanism for damage-related molecular patterns (DAMPs) to induce host immune responses.

Recently, Dai Xiaofeng's team at the Institute of Agricultural Products Processing of the Chinese Academy of Agricultural Sciences made new progress in the study of the interaction between the yellow-wilted pathogen, the Dali-Dora-Dora, and the host plant.
the study, a new mechanism for inducing host immune responses is found in the study of the involvement of Thyme cytase in damage-related molecular patterns (DAMPs).
research published online October 26 in molecular plant-microbial interactions.
Plant cell horning and embolism can resist the invasion of pathogenic bacteria, and pathogenic fungi through the secretion of cuticle enzymes to degrade or embolism, thereby breaking through the physical barrier to achieve infestation.
keratase can also be identified by plant cell surface receptors as an exciter that does not depend on enzyme activity, and participate in the interaction between pathogens and host plants.
the above biological processes occur in the pathogen-infected host plant leaf tissue process, such as rice plague bacteria, gray grape spores and other pathogenic bacteria.
for pathogens that lack the root tissue of the layer, the pathogen usually does not need to use keratase to break through this physical barrier.
, the role of keratin in earth-transmitted pathogen fungi has been unclear.
research team through comparative genomics analysis found that the earth-to-earth pathogen Dalicoli bacteria by coding 13 keratin genes, more than most of the leaf-damaging tissue.
the pathogen fungus has significantly amplification, and multiple gene-response pathogens infest the host process, suggesting that the family expansion of the hornase gene is related to the pathogen infestation process; CBM1)'s enzyme (VdCUT11) can induce immune responses in tobacco, tomatoes, cotton and cause cell necrosis; It was shown that the keratase itself did not act as an exciter, but relied on the enzyme's live degradation product to stimulate the plant's immune response, and the researchers found that the keratase VdCUT11 degradation product relied on the trans-membrane recepor protein complex to induce an immune response, while the disease The original use of carbohydrate binding module CCM1 protein (VdCBM1) to inhibit the above-mentioned immune response, to achieve the host plant invasion, gene knockout, functional retesting and other genetic experiments confirmed that the gene because of the toxic function of dali rota, participated in the invasion process against the host.
the study for the first time found that the use of damage-related molecular patterns (DAMPs) in Dali's branches to participate in the interaction of pathogens and hosts, identified the new functions of keratin enzymes involved in the soil-to-pathogenic fungi intruding root tissue, and confirmed that the cellulose domain (CBM1) both promote enzyme activity and inhibit immune response, providing a theoretical basis for in-depth analysis of the mechanism of the mechanism of the interlocution and host.
the research was jointly completed by the Chinese Academy of Agricultural Sciences Processing Institute and the University of California, Davis, and the first completion unit was the Institute of Agricultural Sciences Processing Institute.
Dai Xiaofeng and Professor Krishna Subbarao of the University of California, Davis, are co-authors.
research has been funded by scientific and technological innovation projects, key research and development programs, the National Natural Science Foundation of China, and special research projects in public welfare industries (agriculture).
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