Deacetyl-Trerophyll Methyl Ester

Deacetyl-Trerophyll Methyl Ester

Deacetyl asperulosidic acid methyl ester

【Structural formula】

[Molecular formula and molecular weight] C ₁₇ H ₂₄ O ₁₁ ; 404

[Source] Hedyotis di ffusa Willd.

【Properties】White crystals

This product is soluble in methanol

Melting point: 129～133℃1

【Purity Check】

TLC

1.

Developing solvent chloroform - methanol - water (5: 1.

Check the 10% sulfuric acid ethanol solution, heat it at 105℃ until the spots are clearly colored, and check under sunlight and ultraviolet light at 366nm

2.

Developing solvent ethyl acetate - methanol - water (9:1:0.

Check the 10% sulfuric acid ethanol solution, heat it at 105℃ until the spots are clearly colored, and check under sunlight and ultraviolet light at 366nm

High performance liquid chromatography

Chromatographic column Phenomenex C ₁₈ column, 5um (4.

Mobile phase acetonitrile-water (7:93), flow rate 0.

Measurement wavelength 236nm

【Structural identification】2381

3320, 1689, 1636, 1441, 1311, 1159, 1098, 1079, 946, 892

FAB-MS m/z: 443[M+K] ⁺ , 405[M+H] ⁺

¹ H-NMR (D ₂ O) δ: 7.

¹³ C-NMR (CD ₅ OD) δ: 101.

[Storage] Store at low temperature
.

Related links: 3,5-O-dicaffeoylquinic acid